Composite

Part:BBa_K5089010

Designed by: Camille Prefontaine   Group: iGEM24_UManitoba   (2024-09-29)


ABO(S146R)-CsgA-LCI

This composite part consists of all components required for expressing and displaying ABO(S146R) and LCI on the cell surface of E.coli BL21(DE3). The characterization of the enzymatic activity of ABO(S146R) is available in the documentation for the base part BBa_K5089008. The characterization of LCI binding ability can be found in the documentation of the base part BBa_K3013000.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 812
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 831
    Illegal NgoMIV site found at 951
    Illegal NgoMIV site found at 977
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None